The Godfather of Surgery

Sisi's body recovered very well. After evaluation, Yang Ping felt that it was possible to use the uncastrated virus for experiments. Although there were certain risks, he decided to give it a try.

If you always stand still and do not take the risk of catching a cold, it is difficult to make some breakthroughs. It is feasible to take risks with full evaluation and preparation.

Even if it fails, just treat it as a serious cold. Yang Ping actively prepares to continue the experiment.

The laboratory of Nandu Medical University began a new round of various tests on the K virus. After multiple repeated tests, the new K virus was considered to have not deviated from the basic characteristics of the source virus, and the safety of using replicable viruses for experiments can be guaranteed.

The tumor laboratory of Sanbo Hospital also concluded through a series of examinations that the K virus currently infects tumor cells mostly and a small number of normal cells. However, from the observation of several experiments, it only causes apoptosis of tumor cells, not normal cells. Its impact on normal cells is lower than that of its source virus-adenovirus on human cells.

The vector source virus used by the K virus is an adenovirus-but not an ordinary adenovirus, but a recombinant adenovirus. Recombinant adenovirus, a replication-defective adenovirus vector system, is widely used in gene therapy, basic life science research and other fields.

Recombinant adenovirus has the following significant advantages: wide infection range, almost all cell lines, primary cells and some tissues can be infected; infection efficiency is as high as 100%, which is far superior to other viral vector tools and liposome transfection; large capacity for exogenous genes, up to 8Kb; no genome integration; high titer and easy operation. Therefore, recombinant adenovirus has always been considered the most promising gene delivery tool.

Of course, Yang Ping borrowed it not to let it deliver genes, but to let it deliver K factor. Before using it, Yang Ping further improved it. Then the K factor was implanted into its protein structure to form a protein complex.

The commonly used adenovirus vector is based on human adenovirus type 5, whose genome is a 36Kb long linear double-stranded DNA. This adenovirus is internalized into the cell through its own fibers and cell surface receptors (such as CAR), and then transferred from the endosome to the cytoplasm and nucleus. With the help of the cell's transcription and translation machinery, the virus replication and assembly are initiated. A complete viral life cycle will cause cell death and release virus particles.

Ordinary adenovirus is not a good thing. Once infected, this virus is likely to cause diseases in the respiratory tract, digestive tract, urinary system, etc., such as pharyngitis, pneumonia, conjunctivitis, etc., causing fever, coughing and sputum, pharyngeal redness and congestion, diarrhea, vomiting and other symptoms. Severe cases can also cause dyspnea and suffocation.

However, the recombinant adenovirus is completely different from the ordinary adenovirus. The difference between them is similar to that between a dog and a wolf. The recombinant adenovirus has been completely tamed by researchers. Not only has its replication gene been knocked out, but also some of its pathogenic genes have been knocked out on the basis of mastering its genes and characteristics. Therefore, the recombinant virus is no longer very pathogenic, but it retains some other characteristics that are useful for experiments, such as: wide infection range, high infection efficiency, and after Yang Ping's modification, it has a very high infection rate for incompletely differentiated tumor cells, and the infection rate for normal cells is lower than that of ordinary viruses.

Therefore, the adenovirus carefully recombined by Yang Ping has great potential. It is a major innovation in itself. It can not only be used in K virus therapy, but also in many future tumor treatments. It is equivalent to adding a high-energy and high-precision weapon to the arsenal of tumor treatment.

Despite this, Yang Ping did not dare to take it lightly. He still carefully re-examined the genes of these viruses to confirm that there was no problem with their genomes.

This special recombinant adenovirus has a huge advantage over the original ordinary adenovirus.

The original common adenovirus has strong infectivity, because it can survive for 30 minutes at 56℃ and for two minutes at 100℃, which provides conditions for its infectivity.

The adenovirus recombined by Yang Ping's team can survive for no more than one minute in a dry environment, and the average survival time is just over 20 seconds. Even in a humid environment, the survival time at 56℃ is no more than one minute. Therefore, this virus is extremely fragile. It cannot survive in an out-of-body environment. It can only exist inside the human body, and the human immune system maintains sufficient lethality to it, which makes it far less threatening to the human body than ordinary adenovirus.

So no matter from which aspect, this recombinant adenovirus is very safe, so Yang Ping's adventure is not random, he has enough confidence to do this.

——

The k virus cultivated by the virology laboratory of Nandu Medical University has been packaged and sent to Sanbo Hospital by cold chain transportation. Yang Ping injected these viruses into Sisi's body.

It is obvious that the live virus has a much greater impact on the human body than the castrated virus. On the third day after the virus injection, Sisi developed a high fever again, but this time the temperature was not so exaggerated, only 39 degrees. For Sisi, who has experienced many tests of a high fever of 42 degrees, he can completely withstand a high fever of 39 degrees.

This time the fever lasted longer than before, but fortunately, it fluctuated around 39.0 degrees, and the highest temperature did not exceed 40 degrees. The lowest temperature was about 38.5 degrees. Its fever pattern was wavy, which was consistent with the situation of the K virus fighting the immune system in the body, because the current K virus was completely different from the previous K virus. The previous K virus knocked out the replication gene. It was a castrated virus. Under the attack of the immune system, its number would only continue to decrease. Therefore, once the high fever passed the peak, it could only go downhill.

The current K virus has the ability to replicate. When the human immune system kills some of the virus, it will restore the number of viruses through replication. In this way, the virus and the human immune system are in a tug-of-war. This tug-of-war is manifested in a wave-like manner in the fever pattern.

The tumor biopsy specimens sent for inspection by puncture were indeed consistent with Yang Ping's speculation. It was obviously different from the previous experiments. The rate of virus infection of tumor cells in this experiment was very high. From the observation of tumor cell specimens obtained by puncture, more than 10% of tumor cells were infected, and this rate was still rising as the virus replicated. The specimens taken from several different parts were extremely balanced.

Due to its dangerousness, intracranial tumors cannot be punctured to obtain specimens, but from the infection rate of tumor tissue specimens in other parts, it is speculated that the possibility of intracranial tumors being infected by viruses is almost the same, because the modified virus can penetrate the blood-brain barrier.

It was this result that made Yang Ping very excited, indicating that after the attack of the human immune system, part of the K virus successfully reached the tumor cells. After reaching the destination, the K virus began to use the nutrients of the tumor to replicate.

Unexpectedly, a cautious adventure would yield such a great harvest. It seems that stability is sometimes not necessarily a good thing. Innovation must have an adventurous spirit.

With continued observation, these infected tumor cells now began to apoptosis on a large scale. From the calculated ratio, almost more than 3% of the tumors died, and the ratio of infection rate to apoptosis remained the same as before, maintaining a ratio of three to one. Because the increase in infection rate directly led to an increase in apoptosis rate.

What does a three percent apoptosis rate mean? This is definitely faster than the growth rate of the tumor. If the treatment continues like this, the tumor has a chance of being cured. I didn't expect that replacing the strict virus with a live virus treatment would bring such a good effect.

At this point in the experiment, Yang Ping also began to think about naming this K virus. In fact, after many modifications, it can no longer be a virus. It must be given a new name-incompletely differentiated cell targeted apoptosis protein complex factor. Yang Ping wrote this thought of name on the whiteboard in his office.

Looking at this high-sounding name, Yang Ping leaned on the back of his office chair to rest. He hadn't been home for these days. Now he should go back to hug his son, and of course, his wife.

——

Sisi's heart examinations showed that the data were better each time than before, indicating that the tumor was still shrinking. The improvement of heart function means that the impact of the intracranial tumor on the brainstem was decreasing. This visible change is more reliable than experimental data. After all, any data in clinical medicine must be converted into clinical changes to be meaningful.

After the virus was injected this time, except for a few days of fever, no other adverse symptoms appeared, which increased the confidence of the experimental team again.

Recalling the beginning of the experiment, it was stumbling, and now everything is under control. Everyone can't help but feel very emotional. Professor Yang is still amazing. His brain is like an alien.

K factor, recombinant adenovirus, the combination of the two, direct clinical trials, experiments with replicable viruses, and the classification of this virus from the virus. It is believed that it should be a replicable protein complex with K factor that can initiate the apoptosis program of tumor cells. Which of these is not an amazing creation.

His experiment looked rough at the beginning, completely out of the blue. It felt too wild. At that time, researchers in the virus laboratory of Nandu Medical University even discussed privately that this idea was impossible to achieve. It was too wild.

However, as the experiment progressed, it became clearer and clearer, and the possibility of realization became greater. Slowly, it was successful. The whole process was a bit incredible.

Moreover, Yang Ping's calmness and rationality in the face of failure also surprised them, because most people would be somewhat hit after failure, but he was not hit at all. He was still full of passion after failure, as if he had already determined that he would succeed.

Reading the error-free version! 6=9+Shu_bar first published this novel.

Yang Ping himself didn't know why he had this talent, and he could always maintain strong passion and curiosity. He relied on intuition and logic to accurately grasp the research direction. Perhaps it was the result of long-term devilish training in the system space before.

Sisi's magnetic resonance image came out again, and Yang Ping measured the tumor volume, especially the tumor in the brain. Not only did it not continue to grow, but it was also decreasing compared to before. Although the reduction was very small, it was much higher than the previous measurement.

The data is consistent with the clinical symptoms, and now we can proceed with the follow-up experiments. As for ethical issues, there is actually a logic of success and failure. When you fail, you will be criticized. When you succeed, others will only see your results and forget your process.

During the rounds, Yang Ping specifically noticed the cactus in the corner. It was getting greener and stronger, and Sisi's energy was much better than before.

——

Masao Fujiwara had a certain friendship with Director Han before. He wrote a letter to Director Han, hoping to help arrange for Miyuki Fujiwara to come to China for long-term further study, which would last for about three years.

The short-term training that Miyuki had here before felt good, so now she wants to have a longer training, hoping to learn Professor Yang's superb medical skills and contribute to the health of Japanese people in the future.

After reading the letter, Director Han thought in his mind that Miyuki Fujiwara met the conditions regardless of her Chinese proficiency or other aspects. Mutual learning is a normal exchange in the world.

But now, Director Han is not familiar with the situation at Yang Ping. Yang Ping has long been the subject leader of an independent research institute, and his current job is just to provide logistical services. Director Han told Yang Ping about the matter and asked Yang Ping to make his own decision.

Yang Ping thought for a while, and those who came to study at this time, whether domestic or foreign, must be treated with caution. He forwarded the matter to Manager Qiu.

Manager Qiu is currently in charge of the entire security work surrounding Yang Ping. Manager Qiu is extremely vigilant about Miyuki Fujiwara's visit. He felt that it might be related to Yang Ping's latest research results. Because Huang Jiacai had told him in advance the importance of Professor Yang's research results, it would likely shake the fundamental interests of many pharmaceutical giants. These people either tried every means to recruit Yang Ping, or they tried their best to steal the research results. If they are unable to do the first two, they may take desperate measures and use extreme measures to stop Yang Ping's research. Bloodthirsty capital can do anything when it goes crazy. Manager Qiu immediately reported the matter to his superiors, who replied: She can come, but she must be used to understand the other party's deployment as soon as possible.

Before coming, Fujiwara Miyuki contacted Xiao Su and Tang Fei. When she was studying in China, the three of them had a very good relationship and were good sisters. They once traveled to Hangzhou together, and this time they came back to say hello to the good sisters in advance.

Regarding Miyuki's return, Yang Ping had no trouble in his heart. On the issue of men and women, his idea was very simple - he would only take a scoop out of 3,000 yuan. He now has a wife and children, and it is impossible to make mistakes in this regard. .

So whether she comes or not, Yang Ping doesn't have much idea. Whether he agrees to her coming or not is left to Manager Qiu to decide.