Start From Emergency Doctor
Chapter 423
Chapter 423
It was too late when Sun Meng wanted to stop Zhou Yuan.
Zhou Yuan is also a surfer all year round. He naturally knows to seize the keywords and directly enter the two words “Zhou Yuan” depression on the search engine, and a lot of results pop up.
The first search result is a Weibo.
“Yanjing Traditional Chinese Medicine emergency doctors, who have been studying hand surgery, suddenly went to study psychiatry. Isn’t this harmful! Who has the ability to be top in two fields?”
“The director of the Department of Orthopedics in our hospital is a national expert and ranks among the top three orthopedic doctors in the country. But even so, he dare not get involved in mental illness, for fear of harming his children and harming patients! But Yanjing Chinese Medicine is a young emergency Doctors, after achieving the achievements in hand surgery, they are complacent and want to cure patients with depression!”
“Not everyone is Leonardo da Vinci, who can be all-rounder! Really new born calves are not afraid of tigers!”
What makes Zhou Yuan find interesting is that the person who posted this Weibo is still a colleague, and the Weibo certification is “Director of the Department of Psychiatry of the Magic City Union Hospital**”.
And the comments below are also somewhat polarized. Most people are not optimistic about Zhou Yuan.
“I am also a doctor, and I know that it is extremely difficult for a doctor to achieve results in a certain field, not to mention two completely unrelated fields of hand surgery and psychiatry!”
The 100** reply is simple: “As a psychiatrist, I think Zhou Yuan is insulting our field of mental illness! Psychiatrists are very hard and their professional knowledge is also very solid, definitely not a layman can interfere! Zhou Yuan is extremely irresponsible to the patient!”
“The medical profession is highly targeted. General internal medicine and general surgery. Just a single internal medicine is divided into dozens of departments, such as respiratory medicine, gastroenterology, cardiovascular medicine, neurology, etc., and a respiratory medicine is also subdivided into colds. , Pneumonia, lung cancer, pulmonary heart disease, respiratory failure, etc., a doctor can be famous all over the country for his achievements in a small profession!”
“For example, Director Wang Wenjiu of our hospital, who has made great achievements in pneumonia, is already a few months away! If anyone says that he can be proficient in multiple medical fields, I definitely don’t believe it!”
There is very little support for Zhou Yuan. Most people think that there are no all-rounders in the world and think that Zhou Yuan is harming others.
There are even people who call Zhou Yuan a “quack doctor.”
This is the biggest insult to a good doctor. He has been a medical student for at least eight years. He has been studying hard day and night in order to have more confidence in treating patients, but these efforts have been denied by a “quack doctor”.
Zhou Yuan’s face was a little ugly, and some netizens even found his personal Weibo and questioned him.
**The message from the verified account is very conspicuous, “You are not an all-rounder! Please don’t harm people!”
Zhou Yuan silently modified his personal information.
“Introduction: All-rounder.”
After the modification, Zhou Yuan put the phone back in his pocket.
Although these remarks affected Zhou Yuan’s mood, Zhou Yuan knew what he was doing.
He would not doubt himself because of this doubt.
At the time of this experiment, Zhou Yuan was the person closest to the new progress of depression!
“Zhou Yuan, are you okay?” Sun Meng asked worriedly.(Read more @ wuxiax.com)
Zhou Yuan shook his head, “It’s nothing. I’m going to do the experiment. If there is an emergency patient, you can call me.”
Sun Meng nodded.
This is not Zhou Yuan’s duty time, and he does not need to sit in the clinic.
Zhou Yuan bid farewell to Sun Meng and walked straight to the laboratory.
At this time, the experiment on the lateral habenula has been going on for more than a week.
“Doctor Zhou.” Seeing Zhou Yuan walk in, someone greeted him.
They really don’t know how to call Zhou Yuan. It is not appropriate to call Zhou Yuan directly. After all, Zhou Yuan is also their leader. It is not reasonable to call Zhou Scholar or Professor Zhou. They can only be called Doctor Zhou.
Zhou Yuan asked: “How is the experiment going?”
“No, I haven’t discovered the specifics of the discharge method of the outer habenula…too difficult!”
Zhou Yuan frowned and came to the test area.
There are eight groups in No. 1 experimental area, two in each cage, and the remaining seven groups have four in each cage. Animal experiments and research have been approved by the Animal Care and Use Committee of the Animal Facility of Yanjing International Hospital, and they also provide experimental animals.
“One group, three groups, five groups, and seven groups are mice with depression, and the remaining four groups are normal mice. We have all administered systemic antidepressants.”
Zhou Yuan asked: “How is the drug administered?”
“According to the method you indicated, all drugs were dissolved in 0.9% saline for intraperitoneal injection, and rats and mice were all given ketamine.”
Zhou Yuan nodded, and said: “Has the in vitro electrophysiology research been carried out?”
The scientific researcher glanced at his watch and said: “No, we are going to check it one hour after the administration… There are still two minutes and twenty seconds.”
“I will do it with you this time.”
The other stages of the experiment have been completed, and now is the most critical time, Zhou Yuan cannot be absent.
Zhou Yuan itself does not have superb experimental ability. What the system gave Zhou Yuan was excellent surgical operation. What he taught Zhou Yuan during the eight years of medical school was only solid theoretical knowledge and basic skills. He had no chance to cultivate scientific research ability .
However, the last stage of the experiment relies on operation!
When I heard that Zhou Yuan was going to do the experiment himself, other people rushed over.
They had heard of Zhou Yuan’s reputation for a long time, and now they can finally witness it with their own eyes!
“Doctor Zhou, time is up.”
Zhou Yuan nodded, “Let’s do the LC-MS measurement of ketamine first.”
Zhou Yuan put on rubber gloves, took out the first group of depression mice, anesthetized the mice with isoflurane, and then decapitated them.
Immediately afterwards, Zhou Yuan started local infusion of drugs.
This is mainly to protect brain tissue. The experiment is to study the lateral habenula of mice, so the integrity of the lateral habenula must be ensured.
After completing the infusion, Zhou Yuan used the brain matrix to dissect the lateral habenula and ventral hippocampus from the brain. After washing with PBS, acetonitrile was added to the tissue grinder at 60hz for homogenization.
After more than an hour of processing, Zhou Yuan completed the preparation of the brain slices and lateral habenular nucleus slices. He connected the isolated brain tissue to the microbial current, and then used the MultiCLAM700B amplifier and pCLAMP10 software for electrophysiological testing.
Series resistance, automatic compensation of capacitance, and soon, the instrument showed a stable gigabit.
Under the stimulation of the current clamp, the isolated brain tissue begins to carry out neuronal activity.
The people in the entire laboratory were quiet, and the scientific researchers in laboratory uniforms stood in front of the instrument, feeling a little nervous.
For three minutes, the value on the instrument is still very stable, and there is no abnormality in the discharge activity.
“Sure enough, it failed this time,” someone said.
“Could it be that mice with depression are not typical enough?”
However, at the next moment, the green straight line on the instrument suddenly fluctuates slightly…
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