doctor knife in hand

The weather is hot, and the fruit will rot after being picked and left for a day or two. This is the most worrying thing for the fruit farmers when they sell it. But I didn’t expect that there are still people who buy the fruit with green hairs. For a while, there are rotten fruits on the whole street. They were all collected in the Duke's Mansion.

If the fruit rots, it will produce a sour smell. The old housekeeper can only find a room a little far away from the main courtyard, and most people dare not go into that room.

"Young Master Lu, that's enough." The old butler said to Lu Jin with a sad face. If Lu Jin hadn't repeatedly emphasized on making new medicines, he would have thought Lu Jin was crazy.

"Enough, let's make a batch first." Then he looked at Song Huai and asked, "Are my bottles ready?"

Song Huai said, "One kiln has already been released, and I can bring it back when the temperature drops in the afternoon."

On the second day, all the utensils and materials were ready.

Lu Jin led his apprentice to put on a mask, changed into clean clothes, and walked into the room with a jar and scraper to collect the green hairs on the fruit.

Although He Lan and Sun Bai had the same doubts as Song Heng, their blind worship of Master convinced them that Lu Jin would be able to turn decay into a miracle in the end, so they worked very hard.

Following Lu Jin requires the spirit of not being afraid of getting dirty or getting tired, so even when you shave your green hair, you have to be careful.

"These green hairs are Penicillium, a very tiny organism. The green hairs we see with the naked eye are the result of tens of thousands and hundreds of millions of Penicillium. What we have to do is to extract from these Penicillium The bacteriostatic substance, penicillin, is the wonder drug against wound infection."

While collecting, Lu Jin explained to the two apprentices.

Microorganisms are very important to modern medicine in later generations, but here, it is still an unknown blank space.

He Lan held up the shaving blade when he heard the words, stared at a small tuft of green hairs on it, and widened his eyes, thinking about the concept of tens of thousands and hundreds of millions.

Ten thousand is already a huge number, tens of thousands is an even bigger number, one hundred million is even unthinkable.

Song Heng stood at the door for a while, rubbed the bridge of his nose, and finally withdrew.

Once the Penicillium is collected, it will be cultivated next.

At this time, the white rice and taro were boiled into juice in the kitchen, and they were poured into thirty petri dishes as culture medium. The petri dishes were porcelain white containers that Song Huai had just brought back, similar to the glass petri dishes of later generations.

Then the Penicillium was grafted and cultured in a Petri dish for seven days.

During this period, according to Lu Jin's instructions, Song Huai and the others had already found a few people with festering wounds in a ruined temple.

Soldiers on the battlefield are mostly injured by sharp weapons, and the wounds are easy to fester, and ordinary herbs can't help.In fact, a large part of the wounded soldiers did not die from excessive blood loss or damage to organs, but that the wounds were slow to heal, and the area became more and more rotten, and finally the whole body invaded the body and died.Of course, if the injury is in the limbs, it can still be amputated, but if it is in a vital point, it will be even more fatal.

So Song Heng went to the ruined temple with Lu Jin, and watched with his own eyes that Lu Jin took pustules from these patients and carefully put them in containers.

If Lu Jin could really develop a medicine to prevent wounds from festering, it would indeed reduce the casualties by half.

"Wounds caused by trauma, and the wounds have suppurated, are usually infected with a small germ. You two should pay attention to the wounds, and you will encounter many in the future."

Lu Jin called two apprentices to come and see.

Sun Bai said, "It's much more serious than those wounds after the operation, master, can they still be saved?"

Lu Jin smiled wryly and shook his head. These are homeless tramps with other illnesses besides wound infection. Now that penicillin has not been extracted, how can we be able to treat these almost sane people?

There were a large number of staphylococci in the pustules, and when Lu Jin returned to the mansion, he cultured the cocci in another medium for the final susceptibility test.

Seven days later, blue and white cloud appeared on the [-] containers for cultivating Penicillium, covering the entire Petri dish.

Song Heng went back to the mansion immediately after leaving court, and several generals of the Song family army followed him, all wanting to see what Lu Jin did.

The room was quite spacious at first, but it was suddenly filled with darkness when people stood still like this.

Lu Jin asked these irrelevant people to stand far away, so as not to interfere with his experiment with his apprentice.

There are funnels and strange clay pots on a long table in the room. The pots are big and small, long and short, and oddly shaped. A pipe is bent out of the bottom and a stopper is plugged.There are also [-] small white porcelain utensils, all of which have white moldy mildew floating on them, which makes people feel hairy.There was a can of canola oil, and another wooden barrel was covered but steaming.

Little Pei was mean, so he lifted the lid nearby and peeked into the barrel secretly, but he couldn't figure out what Wu Qima was doing, so he received a sharp look from the boss, and was dragged back by several brothers to bind his hands and feet in an instant.

Lu Jin knocked on the table and said, "Okay, now we're starting to do experiments, the purpose is to extract the antibacterial substance, penicillin. Irrelevant people should not touch anything on the table casually."

Xiao Pei, who was thrown to the door, sneered at who the last sentence refers to.

"Alan, write down the steps and precautions."

After giving the order, Lu Jin picked up the funnel and stuffed a ball of cotton into the funnel.Then the funnel was placed on a clay pot with a big belly and a small mouth, and a hole extended from the bottom of the pot, which was now tightly closed with a wooden stopper.

Then pour the contents of the thirty long-haired petri dishes into the funnel one by one, and the liquid flows into the clay pot through the cotton ball, while other impurities remain on the funnel.

Lu Jin said, "Penicillin has a characteristic that it is soluble in water, so it is now in the water in this earthen jar."

The apprentices nodded with a half understanding, and the onlookers were even more bewildered.

The newcomers whispered to each other, "What is penicillin?"

They didn't want to disturb Lu Jin, so they moved to Song Heng's side, thinking that the general would always know about it.

Song Hengqing glanced at them indifferently, "Didn't Ah Jin say that the medicine extracted from those long-haired things can prevent wounds from festering."

As for why those hairy things can have this kind of medicine, he doesn't know.Don't look at Song Heng who seems to be taking it very seriously, as if he knows why Lu Jin is doing the steps, but in fact he is also asking why deep down in his heart

Lu Jin didn't bother to explain much. In order to let his apprentices understand deeply, he spent a lot of time explaining, and he almost developed a microscope for them to watch.

Lu Jin took the funnel away and ordered, "Give me the rapeseed oil."

Sun Bai immediately took the can of vegetable oil over.The rapeseed oil was poured into the crock and stirred, then he closed the lid and let it stand for half an hour.

During this period, He Lan asked, "Master, why put rapeseed oil?"

Lu Jin explained, "The only thing we need is penicillin, but we only used cotton to filter the culture solution just now, but we can only filter out the impurities that our eyes can see, that is, the white floating hairs that are still left on the funnel cotton, and the tiny However, it still followed the water into the jar. Rapeseed oil and water are incompatible, so some impurities that cannot be dissolved in water but can be dissolved in oil will be sucked into the rapeseed oil and float on the water, while penicillin will remain stay in the water, so we have initially purified the penicillin."

After Lu Jin finished speaking, everyone around him hummed, not knowing what they were humming.

He Lan wrote furiously, although he didn't understand much, but it's always right to write it down first.

When the time was almost up, Lu Jin placed another clay pot under the spout of the pot, opened the cork, and a stream of clear liquid flowed into the pot.

In order to prevent the oil from mixing, he did not let the clear liquid in the separation pot flow out completely, so he plugged the wooden stopper.

He put the clay pot containing the clear liquid on the table, and said, "Although the water looks clear, there are still other impurities mixed in besides the penicillin. We have to purify it again and give me the barrel of charcoal."

Sun Bai walked around the table, held the smoking wooden barrel, and placed it in front of Lu Jin.

"So it's charcoal inside." Xiao Pei muttered.

Lu Jin opened the lid, dug out the wet charcoal mud inside, and put it into a clay pot. While stirring, he explained, "There are other germs and microbes in the charcoal, which can be killed and eliminated after boiling water, so put penicillin liquid No germs will be brought in. The charcoal has adsorption capacity, it will absorb the penicillin, so we can safely purify the liquid further.”

The more he talked, the more esoteric he became. This time, he couldn't even hear a hum, and only watched him move with his eyes open.

Lu Jin smiled. After stirring evenly, he poured the toner into a long straight porcelain tube with a large top and a narrow bottom. The same porcelain tube still had a water outlet under it, which was plugged with a small wooden stopper.

The pipe couldn't stand upright, so a special wooden shelf was set up, and a pot was still placed under the water outlet. Obviously, liquid would flow out of the mouth later.

Next, Lu Jin picked up the distilled water on the table and poured it from above the porcelain tube.

"This water is purer than boiled water, and there is nothing in it, and this step is to clean the impure substances that come with the toner."

After the water covered the black charcoal, after a while, Lu Jin opened the cork and let the water flow down the outlet to the jar below.

Then he replaced another clean jar under the spout, picked up the vinegar, and continued pouring it into the porcelain tube.

"Penicillin is a weakly acidic substance, while vinegar is more acidic, so it will not dissolve in vinegar, and you can safely wash away alkaline impurities."

Seeing that He Lan stopped writing, with a puzzled face, Lu Jin said, "It's okay if you don't understand, just write it down, and I will take you to do research slowly in the future."

As for the others, it was like listening to the heavenly scriptures, and they didn't dare to utter a word.

While opening the cork to let the vinegar flow out, Lu Jin glanced at Song Heng and couldn't help but smile, because the latter finally couldn't pretend anymore. In this field, he was really powerless.

"At this point, there is almost nothing mixed in this tube of charcoal except penicillin, so the next step is to separate penicillin from the carbon powder."

"Then how to separate?" Sun Bai asked.

Lu Jin picked up the bottle of alkaline water and said, "Use this."

He poured alkaline water into the porcelain tube and explained, "As mentioned earlier, penicillin is a weakly acidic substance. Although it is insoluble in acidic water, it can be dissolved in alkaline water. Similarly, if you don't understand acid and alkali, you should write it down first." .”

After the liquid slowly flowed into the jar, Lu Jin put it on the table, and concluded, "At this point, the extraction work is complete. According to theory, it contains penicillin, a good medicine that can inhibit wound ulceration."

After Lu Jin finished speaking, all eyes were fixed on this humble jar, with admiration, longing, hope, and caution in his eyes.

If it is true what Lu Jin said, the things in this jar can make the world turbulent

It is not an exaggeration to call it a magic medicine.

Song Heng moved his fingers lightly, suppressed the excitement in his heart and asked, "How do you know if it works?"

Lu Jin said, "Before I extracted the pustules of the patients with festering wounds, I kept them all the time. Abai, take out all the fifty petri dishes with pustules on them and put them on the table. We will do an effect experiment soon."

Fifty petri dishes were placed on the table, and when opened, there was a pale yellow paste-like thing inside.

Lu Jin diluted the penicillin with distilled water, asked He Lan to record each concentration, and then took one drop and dropped it into the middle of the corresponding staphylococcus culture medium.

"Penicillin has an antibacterial effect, and we can't see it now. Let's look at it after seven days. If the place where the penicillin solution is dripped in the middle of these petri dishes forms a white ring, which is different from the surrounding light yellow, then it is successful. It kills the bacteria." The germs are dead."

"What if there is no change?" Deputy General Hu asked.

Lu Jin lowered his eyes and said, "That's a failure."

The author has something to say about Pang Qiqi, we all know the characters clearly, but how can we not understand when they are spelled together?

Pei is because you read too little.

General Hu Na has read a lot of poetry and books, do you understand?

Song Heng didn't want to talk, and gave this group of eyes the back of their heads.

s Indigenous method for extracting penicillin refers to Renyi and Baidu search content.

In addition, it is indicated that without modern scientific equipment and technology, it is impossible to rely on those bottles and cans alone

Just look at it and have fun.

Thanks to the little angels who voted for me or irrigated nutrient solution

Thank you for the little angel who threw the landmine, lucky one;

Thanks to the little angels who irrigate the nutrient solution

2335 bottles of Guigui Yameow; south of Sioux City. 2 bottles; 1 bottle of Yanning Yezi, a deer, and Shizuo;

Thank you very much for your support, I will continue to work hard