The daily routine of the ancient imperial examination

After everything was ready, Lu Xiuyuan drove away everyone and stayed in the courtyard of the third room to start the experiment.

The penicillium on the melon was probably not enough, so he put the dozens of steamed buns prepared by Nan Xing in the dark corner where it was most likely to rot and mold, and began to prepare for the next army of penicillium.

In order to cultivate penicillin for human use as soon as possible, Lu Xiuyuan found several medium-sized small bowls, and added boiled potato water and rice juice to them.

Stir evenly and wait for it to cool down, find out the moldy and rotten melons, and use the boiled and sterilized bamboo chopsticks to gently transfer them to each bowl.

Different melons use different petri dishes. When there is no way to analyze the strain type, this is also a way to expand the scope in order to find the real penicillin.

The first step of the culture medium was completed, and seven days later, the surface of the culture medium kept in a specific environment bloomed with blossoming Penicillium clusters.

The cultivation was successful, and then began to filter and extract. There was no filter paper in ancient times, and it could only be replaced by boiled and sterilized multi-layer cotton cloth.

Remove the excess debris from the culture medium, add a large amount of vegetable oil to the many crocks containing the filtrate and start stirring.

Because penicillin is water-soluble, the reason for adding oil is to remove fat-soluble impurities and most of the insoluble substances in the filtrate.

After the inside is stratified, use a sterilized spoon to skim off all the grease on the top, and the rest is all liquid and water-soluble mycin or other substances.

Add an appropriate amount of boiled and sterilized toner to absorb all the liquid at the bottom of the crock.

Because there were no such things as separation columns in ancient times, Nan Xing directly asked the iron master to make several separation columns by hand according to the drawings he gave.

Put the toner that has absorbed the liquid into a special separation column, then wash it with distilled water and acidic water diluted with white vinegar, and finally put it in alkaline water to rinse.

After adding all the liquids that should be added, now wait for them to stand and separate. The penicillium in the charcoal will be concentrated and then released. It will definitely be divided into a certain part of the separator, because the iron separation column cannot see the color layer. , Lu Xiuyuan's next operation can only be based on feeling.

The penicillium on the melon was only enough for him to make ten portions. After the ten portions had been left to stand for a while, he began to pour the liquid from the marked No. 1 separation column into the No. [-] culture medium.

Slowly pour in the appropriate liquid and then seal the mouth, and then continue to pour into the No. 2 culture medium.

When he poured out all the liquid in the No. 8 separation column, the number of the culture medium had reached [-].

Next, repeat No. [-] and No. [-] until all the liquid in the No. [-] separation column is poured out, and the next step is to wait for the culture fluid to start to mold.

After the end, Lu Xiuyuan was not idle. He was worried that if the penicillium in the melon was useless and poisonous mold, wouldn't his work be for nothing?Therefore, I also prepared a lot of steamed buns.

Today is the seventh day, and in about seven days, the results of melon cultivation can be processed in the second step, and after seven days, the mold grown from steamed buns can also be harvested for today's first processing.

After taking off his gloves, Lu Xiuyuan prepared to change into new gloves to pick up the moldy steamed buns and continued to pick up the mold.

It's just that the tense body that was tense before the separation fluid was suddenly relaxed, and his body was a bit unbearable. As soon as he stood up, he felt that his eyes went dark, and all sensations in his body disappeared instantly.

Lu Xiuyuan's first reaction at that time was that the culture fluid was in front of him, so he quickly stretched out his hands to keep his balance and not fall down!

However, the lack of lunch and postural hypotension made the darkness very fierce. He couldn't control his body at all, and finally sat back with force based on feeling.

It's better to fall on the back, try to land on the buttocks first, so that the back of the head and the culture fluid can be preserved.

Unconscious, he didn't know that he didn't fall to the ground, but passed out in Shi Yunchen's arms.

Lu Xiuyuan didn't know how long he had been in a coma, and he was already lying on the bed when he opened his eyes. Feeling the sweet taste in his mouth, he knew that Ah Chen must have fed him sugar.

Raising his arms to let himself lean on the head of the bed, he touched his deflated stomach, and began to call out the door affectionately.

"Achen~ I'm hungry~"

When the voice fell, the outer door just opened, and Shi Yunchen walked in with a tray.

"Achen, what time is it now? How long have I been unconscious?"

"You still have the nerve to say it?" The other party put the wooden tray directly on the bedside table, and the sound of the collision made Lu Xiuyuan's heart skip a beat.

"Anyway, you are also a fifth-rank official in the court. Can you stop forgetting to eat and sleep like this? What's wrong with continuing to work on those things after eating?"

The more Shi Yunchen talked, the more angry he became. Early in the morning, he noticed that Erlang hadn't eaten much. It was already past noon and he hadn't come out of the backyard, so he could only go there secretly to see how he was doing. Just eat lunch first and then do it.

As a result, who would have thought that he would see Lu Xiuyuan's unsteady stand just as soon as he entered the yard.

He picked him up and took him back to his bedroom to rest. He fed him some water and stuffed it with a piece of sugar, and then hurriedly went to cook rice porridge again.Shi Yunchen went from being anxious at the beginning to now angry and depressed after waking up.

It's just that the young master regretted it not long after the murder was over. He also knew that Erlang wanted to research the medicine as soon as possible, but seeing the man fall in front of him, how could he not be indifferent?

When Lu Xiuyuan saw the annoyed look on the other party's face and the red corners of his eyes, he naturally understood something. When he stretched out his hand and grabbed it, the corner of Yun Chen's clothes shook slightly.

"I'm sorry, Chen, this time it's my fault, and I won't be like this in the future."

"Hmph." The other party snorted coldly, and sat down on the edge of the bed.

He directly rubbed his head up: "My dear Achen, that experiment had to be finished in one go because of a bad pause in the middle. This time I miscalculated the time, so it won't happen next time, okay?"

"The body is your own, what do you promise to do with me?" Shi Yunchen turned his head sideways, reached out and took the white porridge on the tray and stuffed it.

"I asked someone to make some light dishes. It's not ready yet. You should drink some porridge to fill your stomach first."

After taking the bowl, he drank it in one gulp, and the other party found a handkerchief from the side to help Lu Xiuyuan wipe his mouth.

He wanted to snatch it over and wipe it himself, but seeing the young master's concerned expression, he moved his fingers and retracted under the blanket.

"Erlang, go to bed first, I'll call you when the meal is ready."

"Hey, no need." Lu Xiuyuan sat up quickly, "I'm fine now, so I don't need to rest."

This is the case with hypoglycemia and orthostatic hypotension. Don't look at the ferocious onset, but the symptoms will disappear after the body adjusts.

Seeing that he still wanted to get up, Shi Yunchen gave him a sideways look: "You don't want to continue messing with your penicillium?"

Lu Xiuyuan smiled at the other party: "The mold on those dozens of steamed buns can be picked and cultivated, and it will end soon."

Hearing this, the other party was a little tangled, after all, Lu Xiuyuan had just woken up not long ago, and he must be worried about going to work on those things soon after he didn't eat.

Seeing this, he simply said: "Since Ah Chen is worried, why don't I direct you to do it?"

"Hey!" Shi Yunchen obviously didn't expect him to say that, he was stunned for a moment, and asked back.

"Can I?"

Isn't that thing complicated?I saw that Erlang had to wear a lot of things before, and there were many precautions.

"Of course!" Lu Xiuyuan replied without hesitation. After he cultivated the first generation of penicillin, he will have to purify it. If he wants to put it into production in the future, he must pass on the method to the people around him.

Right now, I can borrow the mold on the steamed buns to teach the young master from beginning to end.

"Then what should I do if I fail?" At this point, the other party was intimidated, "Why don't you do it, and I'll watch over you by the side."

Lu Xiuyuan simply got up and walked towards the backyard with his arms around him, and said as he walked, "It's okay, everyone has a first time, just get used to it."

The two came to the third entrance. First, they washed their hands with running water. There was no disinfectant here. They could only wash it twice with acacia. They stepped on the button below, opened the iron bucket lid with a chain, and took two pieces of disinfectant inside. Wipe your hands clean with the poisoned square towel.

Shi Yunchen put on gloves, took the moldy steamed bun he handed over, and carefully fiddled with the mold on it into the culture solution.

These operations are actually not difficult, even simple enough to be a bit boring, but the other party is still very happy. This has nothing to do with the experimental operation, but the sense of participation.

Instead of standing outside and watching Erlang do it, he could do it with Erlang himself, which made Shi Yunchen extremely happy.

After cleaning up the penicillium on all the steamed buns, I also made ten copies of the culture solution this time, and left the ventilation holes, and then put these specimens labeled [-] into the rotten melon specimens.

After the work was over, Lu Xiuyuan taught the other party the seven-step hand washing method again. The young master had already memorized the steps, but seeing his serious teaching expression, the little devil in his heart quietly surfaced.

The sly eyes turned slightly, and then directly sprinkled the water drops from his hand on the person next to him when he was not prepared.

After playing the prank, he ran away. Lu Xiuyuan wiped a handful of water from his hair, took a handful of water and chased after him.

"Well, you Chen, you dare to plot against me!"

When Shi Yunchen heard the footsteps chasing after him, he couldn't help but look back, and at a glance, the other party noticed the handful of water in his hand.

If this is splashed on the clothes, it will definitely be wet!

"Erlang, you fouled me! I didn't tell you so much!"

"Hmph~" Lu Xiuyuan snorted coldly, did he realize that he was afraid now?

He ran two steps faster and grabbed the person directly. The two staggered and fell to the soft grass.

After two laps following the inertia, Lu Xiuyuan successfully pushed the man under him: "You are always naughty, and today I will teach you a profound lesson as a husband!"

Shi Yunchen lay on his back on the grass, opened his eyes and looked at the person who spoke harshly in front of him, and couldn't help laughing out loud.

His family's Erlang is really stupid, how can he say harsh words with a smile, it sounds like there is no threat at all.

The author has something to say: the penicillin extraction method refers to Baidu and I processed it myself.