Rebirth of the God-level Xueba

Making primers is a very difficult task. Perhaps it is not as difficult as using "seven or forty-nine virgins as drug primers", but it is also more difficult than the rootless water from the desert.

In the era after the rapid development of computer technology, biologists specially developed special software in order to make primers, such as powerful and easy-to-use pre, common, or vectorntisuit, dnasisomiga, dnastar, etc., just look at the number of software It can be thought of how complicated this is. As for the version number, it can better explain that these softwares have not solved all the problems.

In fact, even though PCR has been invented for 30 years, designing primers is still not a routine science. Of course, scholars have summed up some rules, but uncertainty and contingency still plague scholars.

Yang Rui did not require working hours in the early stage of the establishment of the genetic engineering laboratory. Now, he has sacrificed 110 hours of magic weapon again because of this uncertainty and contingency.

Uncertainty and contingency are summed up as probability, and the most natural way to solve probability problems is to increase the sample.

Researchers in the entire genetic engineering laboratory have worked hard to complete the work of primer design.

Publishing papers in first-class Chinese journals is Yang Rui's reward for them, and accordingly, hard work is inevitable.

At this point, Yang Rui is very open-minded.

The gap between Chinese laboratories and laboratories in developed countries is huge. From funding to instruments, from management to technology, from environment to accumulation-the reason for still being able to make world-class results is the only thing that can fill these huge gaps. It is the blood and tears of experimental dogs and researchers.

Modern laboratories have access control systems. Whether it is punching cards, fingerprints, or iris, they can record the working hours of experimental dogs and scientific researchers. If the Chinese laboratories and foreign laboratories Comparing the laboratory attendance time, you can clearly see the gap.

Most foreign laboratories have an eight-hour work system. The more developed the laboratories, the more fixed the working hours, and if you visit domestic laboratories-Tsinghua's biological laboratories, doctoral students attend work every month The time is about 450 hours.

Attendance for 450 hours a month is much harder than 110 hours a week. This means that PhD students at Tsinghua University will not have more than 3 days of rest per month, and it is impossible for them to sleep 8 hours a day. Moreover, in addition to eating and sleeping, the experimental dogs must devote all their time to the laboratory.

If science needs sacrifices, its sacrifices are the time, blood, tears, and hardship of the experimental dogs and researchers.

A large part of the reason why Yang Rui did not continue his studies after completing his master's studies is that he can withstand the consumption of time and blood and tears, but it is difficult for him to endure the hardships in studying.

Doctoral students with an average age of over 25 are already standard adults. However, 450 hours of work per month can only earn about 1,200 yuan. It is hard to say that it is hard.

Once Yang Rui struggled hard, still unable to escape from the quagmire of real life-when you returned to the dormitory after a hard day, exhausted, hungry and thirsty, picking up a box of luncheon meat, but reluctant to open it, study The aura of his success and academic dedication has faded.

It was also because of this experience that the first thing Yang Rui did when he returned to the 1980s was to solve his economic difficulties.

And when the economic dilemma was resolved, Yang Rui no longer had any scruples——

Even if he is faced with the daunting and difficult primer design, it is no exception.

To be honest, in this work, Yang Rui can get very limited help from the database in his mind. He can at most get some theoretical guidance.

Of course, it is commendable to have theoretical support. The theoretical guidance not only pointed out the direction for Yang Rui and narrowed the scope of experimentation, but also gave him great confidence.

Even so, the actual operation is still difficult.

At least it is many times more difficult than that of scholars 30 years later to make unknown primers.

Scholars of later generations have at least primer design software available, only this one does not know how much time to save. Later scholars have more templates for reference...

In 1985, Yang Rui couldn't even pursue the efficiency of the instrument.

However, Yang Rui was not at all afraid of difficulties.

He is full and warm, has beef to eat, has custom-made windbreakers, wants to ride a car, wants to ride a bicycle, he also saves two houses of stamps, has his own laboratory and valuable patented technology.

Even if all the time and energy are devoted to primer design, even if it takes months or even years, Yang Rui does not feel daunted.

What's more, he is not working alone.

After all, the genetic engineering laboratory is in charge of Yang Rui, and the eight researchers in the genetic engineering laboratory are also valuable helpers.

The scholars who do primer design in later generations are not good. Many biological experts have experience in primer design. Indeed, they have more technical means and materials to back up, but scholars who do this kind of research can have an assistant even if it is good. , I don’t have to think about it if I want 8 professional researchers to devote 110 hours a week to help.

After all, the **** of science heard Yang Rui's prayer, and after sucking the blood, tears and time of eight researchers, he gave back to the genetic engineering laboratory, and slightly made up for the shortcomings in technology and equipment.

The only thing Yang Rui can guarantee is that his fellow researchers will not suffer hardship.

Although he can't give extra salary to the researchers in public laboratories, it is always simple to learn more benefits like the current state-owned enterprises.

Companies like China Animal Husbandry are also willing to meet Yang Rui's requirements.

Taking advantage of the time the researchers were around the experiment table, Yang Rui made a few phone calls, turned around, and smiled: "I reported my good news to Director Mei, Director Tie, and Director Qu. After the agreement, I invite them to come. Demonstration. In addition, Director Mei promised to provide us with another cow as a gift. Of course, it was slaughtered."

The researchers in the laboratory all laughed.

"Director Mei is a good man, and he sent beef."

"We haven't finished the beef last time in our house."

"You don't know how to eat, I will tell you, China Animal Husbandry sends fat cows. Don't just cook it. You can use the oven to eat. It's the stove that we use in winter. Does your family have an oven?"

When it comes to eating, the atmosphere on the experimental table suddenly becomes harmonious.

Yang Rui was also smiling, and when several people read the experimental records in turn, he signaled to turn on the electrophoresis apparatus again.

The primers are invisible to the naked eye. Don’t expect a weaker microscope. Moreover, you can’t judge actg even if you see them. Therefore, the way to verify the success of the primers is gel imaging. Run electrophoresis.

Electrophoresis can not only see whether the primers meet the requirements, but also see how effective the primers are, which is the so-called specificity.

Yang Rui had done it just now, and at this time, he handed the work to Gu Qiang and asked him to check it again.

Gu Qiang's experimental operation was not good enough. The accuracy was an order of magnitude higher than Yang Rui's. It was not exaggerated. Everyone believed in his operation more. They watched Gu Qiang's movements one by one, and became nervous again.

Yang Rui was also a little nervous inexplicably.

False positive data in the laboratory can’t be more normal. Materials are contaminated, or operating errors, and so on. The famous penicillin is actually the petri dish used in the experiment is contaminated, and it falls in some laboratories on the spot. Some experimental dogs must be hung up on the altar to let blood.

In this primer design, although Yang Rui used the data in his mind, there were no specific details at all.

Therefore, the primers that Gu Qiang wants to test now are equivalent to those designed by Yang Rui independently.

This effort, even after 20 years, is better than ordinary doctor-level head dogs. However, the independent design means that Yang Rui can't compare the data and confirm it again. At this time, he can't help but feel a little uneasy.

If after Gu Qiang did gel imaging, the specific band of the product's electropherogram was not obvious, or there was a dimer band, it would be a shame.

However, shame is also ashamed, and there are always times when doing scientific research.

Yang Rui watched Gu Qiang operating the electrophoresis apparatus very rhythmically, but slowly relaxed.

Speaking of it, when he first went to Professor Tang Ji’s laboratory, what he did most every day was the electrophoresis instrument. To say that the operation was not so subtle, it was not easy to make mistakes.

Gu Qiang's operation is naturally better. Just looking at his movements gives people a clean and tidy feeling.

When the electropherogram of the PCR product comes out, the band is clear...

However, everyone's focus is only on that specific band.

"The output is so high..." Several people applauded.

The bands displayed in the electrophoresis of the PCR product are a dark "barcode", and the "barcode" is white ~www.wuxiaspot.com~, which are long and short, arranged in sequence.

The clearer the bar code strip, the more the content of the substance.

As far as the current experiment is concerned, the clearer the specific band, the better, and the less dimer or hairpin structure the better.

Yang Rui couldn't help but raise his head up. This was completely his design.

"Professor Ouyang, let's do a set of appraisals?" Yang Rui's self-confidence greatly increased.

Ouyang Shi responded loudly.

Sun Zhengchu was envious when he heard it, and said: "This experiment is done, I'm afraid it can be published in top foreign animal husbandry journals. Chinese Journal of Nutrition () or British Journal of Nutrition?"

Yang Rui smiled without saying a word. Although the Journal of Nutrition is the top journal of animal husbandry, it is not the top choice for him.

"Look at the results of the experiment first." Yang Rui didn't say much, and returned to the state of experimenting.

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