I really don't want to lie down and win

"..."

"Professor Xu, why don't you go back to the guest house first? Academician Zhong asked me to take you to the guest house." The staff member smiled bitterly: "You just got off the plane, you should take a rest first, tomorrow... you may be a little bit late tomorrow. busy."

"Oh..."

soon,

Xu Mang went to the so-called guest house. In fact, it was the place he had been to last time. It might be because of the upcoming Chinese New Year. Xu Mang soon checked in. When he came to his room, he didn't feel sleepy at all. He took it out of his handbag. He took out his notebook and continued to look at the data.

Logically speaking,

Even if it is not the upgraded CRISPR Cas9 system, it should have higher target accuracy, but why is the error rate of the upgraded CRISPR Cas9 system [-]%?

"Ugh..."

"I'm going to die!" Lying on the bed, Xu Mang looked at the ceiling above his head. For a moment, emotions that he couldn't let go gradually came to his mind. He really had no idea. It just opened up Pandora.

Unconsciously,

Xu Mang fell asleep until four hours later, he was woken up by a phone call.

"Hey?"

"Well...I'll be right over." Xu Mang yelled, washed up in a daze, and then went to the Institute of Biochemistry and Cell Biology of the Academy of Sciences.

quickly,

Xu Mang met Academician Zhong, the head of the Institute of Biochemistry and Cell Biology of the Academy of Sciences, but he was in poor condition, his eyes were slightly bloodshot, it seemed that he didn't sleep well yesterday, thinking about it... this happened How could it be possible to sleep well.

"Professor Xu Mang."

"I finally waited for you." Academician Zhong said with a wry smile: "The gap is too big, almost your AI calculation results are all wrong, and it is a very serious off-target error, Professor Xu Mang...what the hell is this? Condition?"

Xu Mang shook his head, and said helplessly, "I don't know either, so I hurried over here to find out the situation."

At this time,

Academician Chu walked in, was stunned for a moment when he saw Xu Mang, and hurriedly asked, "Professor Xu Mang?"

"This is Old Chu, Academician Chu." Academician Zhong introduced.

"Hello, Academician Chu."

After a brief exchange of pleasantries, the three of them began to talk about the root of the problem, but after ten minutes, they still had no clue, but the most embarrassing one was Xu Mang, after all, all the problems were on him.

Immediately afterwards, Xu Mang came to the laboratory, got a more detailed information report, and then applied to Academician Zhong for a temporary office.

"Professor Xu Mang?"

"Have you found any problems?" Academician Zhong asked.

"Not yet." Xu Mang still shook his head, and said helplessly, "But academician Zhong, don't worry... Since I promised to help you upgrade the CRISPR Cas9 system, I will definitely do it. By the way... can you give I have a key to this office?"

"Uh?"

"Professor Xu Mang?"

"Aren't you going back?" Academician Zhong asked.

"Ok!"

"Pride won't let me go back." Xu Mang said seriously, "This matter can't be settled, and I feel very uncomfortable."

For a time,

Academician Zhong didn't know what to say. He had heard from his old friend, Xu Mang's second grandfather, that this guy had an unusual head, and he couldn't pull ten cows back when he became stubborn. Mixed with some water, who would have thought... it's all true.

"Professor Xu Mang."

"It looks like the Chinese New Year is coming, why don't we do it after the next year." Academician Zhong said.

"never mind..."

"I also lock myself up at home. It's better to be here than to go back with troubles." Xu Mang smiled wryly, "It's better to be alone than to be happy, but it's better to be alone than to be sad. Academician Zhong... you Tell the researchers in the institute, come back during the Chinese New Year... I will give them a satisfactory answer and let them relax."

Hearing Xu Mang's words,

Academician Zhong knew that he planned to take all the responsibility alone.

...

There are still three days until the New Year's Eve,

The Institute of Biochemistry and Cell Biology of the Chinese Academy of Sciences was empty. Except for those who were on duty, almost everyone went home.

During these few days,

Xu Mang was not idle. He found the problem. The upgraded CRISPR system should not be edited by a nuclease like Cas9. It needs a smaller nuclease to cut a DNA strand, and then The reverse enzyme is guided by the RNA strand to perform the task of gene editing at the cut site.

at this time,

Xu Mang wanted to find a brand new pair of genetic scissors. The brand new RNA-guided nuclease needed to have three characteristics, smaller, more stable, and more complete.

Smaller means that it is easier to be used in clinical gene therapy that requires in vivo delivery, more stable means that it can maintain high enzyme activity in a wide temperature range and pH range, and safer means that there are fewer off-target effects.

but...

This is not so difficult!

in fact,

Xu Mang has two brand new RNA-guided nucleases in his hands, namely Cpf1 and C2c1. These two proteins are smaller than Cas9 and easier to enter tissues and cells, especially Cpf1, which is far away from the recognition site when it cuts. Far, giving researchers more options in choosing where to edit.

However, Xu Mang prefers C2c1, an RNA-guided nuclease, which is smaller than Cpf1 and is easier to deliver between cells through viral vectors. However, C2c1 is thermophilic and difficult to develop into a useful gene editing tool. .

no way,

C2c1 comes from acidophilic thermostable bacteria, which need a certain temperature to operate normally. According to a foreign research report, its optimal temperature is 48 degrees Celsius, which can directly burn ordinary mammals to death due to high fever.

Redesigning the temperature of C2c1 was a challenge for Xu Mang. As long as he completed this challenge, all the following problems would be solved easily.

At this time,

The phone rang.

The caller is uncle.

"Hey?"