I Don’t Want to Win Lying Down

hard,

Almost unlikely.

After the three young postdoctoral fellows saw this information, they retreated immediately. After all, Xu Mang's proposal to solve the problem of replacing a certain DNA fragment with a target sequence is a false proposition in the world. It does exist, but it cannot be solved. be realized.

Do you think it's difficult? Seeing the expressions of the three, Xu Mang knew that they might think they were crazy for making such a request, but these requests were made by others, and he said helplessly, This is a scientific question. The request issued by the court, so... let's do our best.

Suddenly,

The three of them didn't say anything, and silently accepted the difficulty in front of them that couldn't be completed. If it wasn't Xu Mang standing in front of them, they might have left, but if Xu Mang was standing in front of them, they felt that there might be a chance.

Professor Xu?

“What approach do we take?” asked a postdoc. “Is it the same AI model as before? Using artificial intelligence to give new life to gene editing research?”

Xu Mang shook his head, and said calmly: I don't know... Maybe, but first we need to complete the theoretical framework for the problem of replacing a certain DNA fragment with the target sequence, and this problem is not the only problem we need to solve. There will always be new problems.”

And new ones?

this...

Replaced with a target sequence problem that was already insanely hard, only to be told it was just one of many.

finally got it working,

However, it never occurred to him that the so-called starting work was to do experiments repeatedly, from the CRISPR-Cas9 system, and only record the process of each failure. Xu Mang was responsible for checking the process of these errors.

no way,

This was the best way Xu Mang could think of. Through the comparison of the wrong process, he could find an entry point. It was stable for a day or two, but as time passed, everyone's enthusiasm was gradually drained away. Like a picture of the walking dead.

this day,

Less than five days before the school holidays,

Xiao Chen, a postdoctoral fellow, came to the laboratory. Today, he was going to ask Xu Mang for leave in advance. The Spring Festival was coming soon, and he had to go home early to avoid the peak period of Spring Festival travel. However, when he told the other two colleagues what he thought, , only to find that...the three of them had the same idea.

???

???

???

Xiao Chen said with a confused face: The three of us... are we going to go back? What about this... What about this project? Let Professor Xu Mang stay in the laboratory alone? Although he is half a man from Shanghai, But is it too cruel to him?

Then what should we do? Xiao Shen said with a wry smile, I have to go home, right?

yes.

After all, it's the Spring Festival, so I should go back. I haven't been back for a year. Xiaoqian, a postdoctoral fellow, said, Actually, this problem... won't be solved for a while. We've been working on it for a week, and we're still standing still. Why don't we relax? Change your mood.

At this time,

Xu Mang carried a bag of fried buns and two bags of soy milk, and slowly came to the laboratory. Seeing the three of them sitting and chatting together, he asked with a smile, Have you had breakfast? I bought a lot of fried buns. Shall we eat some together?

Seeing Xu Mang's arrival,

The three of them didn't know how to speak for a moment, and finally Xiao Chen said: Professor Xu...I plan to ask you for leave today. I want to go home early to avoid the army of Spring Festival travel. I haven't been back for a year...

Professor Xu, I also want to ask for leave.

Me too.

Facing the three people's request for leave, Xu Mang was not surprised, and nodded with a smile: All approved, of course I have to go back for the Chinese New Year, by the way...

words fall,

Xu Mang took out three red envelopes from his satchel, handed them to the three of them, and said seriously, I planned to give them to you later, but since you are going home today, I will give you the red envelopes now. .

Suddenly receiving Xu Mang's red envelope, the three of them were surprised for a while, but even more surprised later, the red envelope was a bit exaggeratedly thick, and it was a full 10,000 yuan.

this...

Professor Xu?

The thickness of the red envelope is a little exaggerated! Xiao Chen said with a serious face: We can't accept it.

Take it.

I don't know how much money is in it. My wife prepared it for me. Xu Mang said calmly, Take it, take it, it's okay... Who dares to talk nonsense, let him come to me directly, and I'll confront you face to face.

Looking at the three people who parted,

Xu Mang returned to the empty laboratory again. Perhaps this was his own destiny, his destiny to fight alone. He sat in the office, smoking a cigarette silently, thinking about the future, and how to face failure or success.

The problem of replacing a certain DNA fragment with a target sequence has been studied for more than a week. I wandered around in a maze and could not find any way out. The process of seeking the truth has been so bumpy, and the reality is even more cruel.

After thinking about it,

Xu Mang decided to go outside for a walk. Staying in the office for a long time would cause his nerves to collapse.

in the school,

Xu Mang walked all the way to the school gate, and saw that many students were ready to go home, but the scene in front of him made Xu Mang a little melancholy. He came to a coffee shop that was not closed, ordered a cup of coffee for himself, and sat in a window by the window. position, looking at all kinds of people on the side of the road.

perhaps,

On the day of birth, everyone does not know, in fact, this is an unreasonable world.

Some people's dream is the stars and the sea, some people's dream is a house, some people are born with talent, some people are struggling with sweat...you can't lose at the starting line, but some people are always standing at the finish line .

This is the reality, this is the reality with a huge gap, and finally accept the setting that I am just an ordinary person.

Resigned?

However...

What Xu Mang saw was hard work. Living in this unreasonable world required a lot of courage.

Hello?

Wife?

I'm in...the coffee shop. Xu Mang received a call from Xiaoman, and said with a smile: No...everyone has gone home for the New Year, and I'm the only old man left in the lab, so what can I do? What should I do? I can only sit outside and drink coffee.

...

Speaking of which, go home. Yang Xiaoman suddenly fell into deep thought, and said seriously: Husband...will you go back to your house on New Year's Eve this year, and then go back to Shanghai on the third day of the Lunar New Year?

Um!

At this time,

Yang Xiaoman asked creakingly, What gift should I buy?

unnecessary.

It's fine for my parents to see you coming. They don't need any gifts. Xu Mang said calmly, How about...buy some jewelry and get some tea for my dad.

Um!

I'll do it right away.

Snapped,

hang up directly,

Xu Mang just finished drinking a cup of coffee, and returned to the laboratory silently, and started his day without a clue. It was not until noon that Xu Mang woke up from this muddled state. After eating a not-so-delicious lunch, he continued to start. In the afternoon, I was out of my mind.

Boil, boil,

This day was just like this being confused by Xu Mang, and he was really helpless. He originally wanted to rely on the system to solve it, but found that this time the system restricted him, so he could only solve it by himself. What's more, he was careless. A task was triggered and it was required to be resolved before the Chinese New Year.

...

What the hell?

Why... why is there no one there?

morning rejuvenation,

Xu Mang was on the way to the laboratory when he found that the school was empty.

Sitting on a chair in the office, he took out the experimental data of the previous three postdoctoral fellows. This was the N+1th time for repeated comparisons. It's just a habit.

perhaps...

My thinking is wrong. Xu Mang frowned, took out his notebook from his satchel, connected to the Internet, and opened the database of the Institute of Biochemistry and Cell Biology of the Academy of Sciences. Xu Mang planned to replace the DNA fragment with The target sequence questions are collected centrally.

well?

Wait a moment!

Why do I keep staring at the problem of replacing DNA fragments with target sequences? Xu Mang suddenly came to his senses. He found that he was a bit too stubborn on the issue of replacing DNA fragments with target sequences. This problem can be completely bypassed. past.

An immature idea came out, first cut a DNA strand and insert the target sequence Y, then the original sequence was excised, another unedited DNA strand was cut, and finally the CRISPR system repaired the cut DNA strand.

This is Xu Mang's preliminary idea. Like the CRISPR–Cas9 system, Cas9 is used to recognize a specific DNA sequence, but Cas9 has been modified to cut only one DNA strand, and then the reverse transcriptase is guided by the RNA strand at the cut site. gene editing.

Yes!

I'm starting to feel it! Xu Mang suddenly gained a new life, but this feeling of new life lasted for only ten seconds, and then fell silent in an instant. If you follow this line of thinking, it seems that a lot of research and experiments are needed, but I don't know how to study biology.

Looking for Xiaoman?

Xiaoman majors in economics, but knows how to do biological experiments.

what to do?

How about... looking for Academician Zhong? Xu Mang took out his mobile phone, dialed the number and called.

beep,

yelled,

Xu Mang hung up directly.

Shouldn't this be the time for 'Xu Man System No. 2' to debut?

...

night,

always come quietly,

Yang Xiaoman had just thrown all his clothes into the washing machine and was about to go back to the living room to watch TV, only to find that Xu Mang, who should have been paralyzed on the sofa, had disappeared.

what's the situation?

Where's this guy? Yang Xiaoman frowned, thinking that he seemed to be struggling with the progress of scientific research recently, could it be...has found a solution to the problem?

Tiptoing to the door of Xu Mang's study room, he gently opened a crack in the door. Through the tiny crack, Yang Xiaoman found Xu Mang sitting in front of the computer, typing on the keyboard in a serious manner. After a while, he lowered his head and picked up the pen again. On paper don't know what is being calculated.

Xiaoman closed the door silently, with a hint of love in her helplessness.

Grinding villain!

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