Auto chess in the operating room

Chapter 324 Allowed to enter the laboratory

"3-0 line."

Tang Lou directly pressed the gap with his hands, stitched while pressing, and quickly completed partial non-invasive intermittent sutures.

"Spacer.3-0 Suture"

Because the patient's lacerated part of the myocardial wound was not neat enough, Tanglou directly used gaskets and then used mattress sutures.

Tang Xianhu was dazzled by the changeable and appropriate suture technique.

"Supplementary blood volume quickly establishes extracorporeal circulation."

Professor Liu was very experienced and began to direct Deputy Director Qian to perform the operation simultaneously. It must be said that with Tanglou's fast and perfect suturing, an originally difficult heart rupture repair operation was as simple as suturing an ordinary limb wound. Generally relaxed.

After 10 minutes, the whole operation was perfectly completed.

"Closely observe blood pressure, heart rate and chest drainage."

"Properly replenish blood volume."

"Maintain water and electrolyte balance."

"Pay attention to the use of antibiotics and tetanus antitoxin to prevent infection."

After Professor Liu left Deputy Director Qian with the postoperative precautions, he announced the successful completion of the entire operation.

Tang Xianhu nodded in satisfaction, of course he understood how powerful Tang Lou's technique was with his eyesight.

After Tang Lou and Professor Liu finished changing and washing, since Dean Tang had a lot to do, they had a conversation directly in the corridor outside the operating room.

Dean Tang said very satisfied: "Professor Liu, Dr. Tang, first of all, I would like to thank you two for my nephew. The operation was very successful."

Professor Liu and Tang Lou naturally said that they were both doing their jobs and they were very honored to be able to operate on the dean's nephew.

"Professor Liu, after my consideration of Dr. Tang's topic, there is no problem at all. You can arrange the opening report according to the normal time node, and I will attend it later. At the same time, Dr. Tang entered the laboratory in advance for ICR The induction of mice, I approve."

"Really, that's a big thank you to the dean."

Tang Lou did not expect that Dean Tang agreed so readily.

"Good job."

Dean Tang encouraged Tang Lou again, and rushed to attend a meeting.

For this reason, Professor Liu also specially instructed some precautions in the laboratory. As for the rest of the laboratory's specifications, of course Liu Qiqi will help.

With the authorization of Dean Tang and the arrangement of Professor Liu, Tang Lou entered the laboratory the next day.

Zijingang University's laboratories are equipped with state-of-the-art equipment and various equipment.

At Tang Lou's request, Wu Qingguo was also very cooperative and sent the first batch of regular Kunming mice needed by Tang Lou to the laboratory.

Although in the system laboratory, Tang Lou has already verified the best induction plan, but as a real experiment, in order to be convincing and credible, Tang Lou still designed the control group and the experimental group according to the normal process , and established a statistical analysis model.

As for equipment, Tanglou also prepared in advance the NTS-1300 constant temperature oscillating water tank from Tokyo Rika Instruments Co., Ltd. and the CX-41 optical microscope from Olympus through the help of Liu Qiqi.

As for the reagents, Tanglou also strictly followed the test procedures and made detailed records. Obviously, this was a very huge workload.

Of course, these reagents and materials were all paid for by Tang Lou himself.

Professor Liu only allowed him to use various laboratory equipment.

After all, Tang Lou's project has not yet started. According to Tang Lou's own thinking, there is no project funding. Professor Liu even persuaded Tang Lou to apply for funding, but Tang Lou rejected it.

After all, for the sake of his big plan, Tang Lou is willing to give up the money, although it hurts a lot!

Tang Lou really wants to become a medical cosmetologist. After all, big U resection, double eyelid surgery, and rhinoplasty are all low-risk and high-yield, especially his textbook-level basic skills. As long as Tang Lou is willing, Those celebrities and big names didn't come here one by one.

Before, Jiang Shu had intended to have a tightening operation, and Yang Jinli had also asked if Tang Lou could do bear surgery, not to mention the latest version of zs, who was undergoing plastic surgery every day, the sharp one could be used to scratch glass out.

Of course, Tang Lou wanted to give her the whole brain.

Tang Lou began to prepare the solvent 0.9% sodium chloride injection very carefully, and the positive reagent CP (purity ≥ purity 1) was purchased from Sigma Aldrich.

The preparation concentration was 4mg/m L; fetal calf serum (fetal calf serum, FBS) was purchased from GIBCO company
Potassium chloride (KCl) was purchased from Sigma Aldrich
Use deionized water to prepare 0.075 mol/L. Giemsa dye was purchased from Sigma Aldrich,

以及最重要的临染色前1份Giemsa原液经9份1/15 mol/L磷酸盐缓冲液(1/15 mol/L Na2HPO4溶液和1/15 mol/L KH2PO4溶液1∶1混合，pH6.8)稀释配制为10%Giemsa应用液。

Looking at these reagents purchased from companies all over the world, Tang Lou was heartbroken again. Fortunately, he had already mastered the final optimal ratio. A lot of reagents and time are wasted, and it may not be successful.

After preparing the reagents, Tang Lou began to take out the mice that Wu Qingguo had sorted in advance.

Each batch of Tang Lou controls 36 mice. These mice are all male at the age of 7-8 weeks, weighing 30-32g.

Although Tang Lou thinks that these mice are not in the best condition, they are from the animal experiment base of the Jin Group after all. These mice have passed the ethical review of the Experimental Animal Welfare Ethics Committee of the National Center for Drug Safety Evaluation and Testing.

At the same time, it also has an animal production license, so Tang Lou's experiment is very rigorous and formal in all aspects, and it can be included in the official paper later.

Although Tang Lou did not graduate from a top medical school, he graduated from 211 anyway, and is very familiar with the basic skills of experiments.

Tang Lou used TOXSTAT2006 statistical analysis software for random grouping to control individual differences in grouping.

After that, clinical symptom observation and body weight measurement were strictly followed the formal procedures.

Since it is in a real laboratory, there is no double-speed function. Tang Lou can only administer medicines honestly, once a day in the morning and afternoon, and observe in separate cages, including whether he is dead, dying, activity status, appearance and being trapped. Hair, trauma, feces, etc.; animals were weighed before administration, during administration and before dissection.

In the next week, Tang Lou entered into a very fulfilling experiment.

Although the results are already known, in order to maintain a rigorous database later, Tang Lou still analyzed and recorded the relationship between different dosage concentrations and the types of chromosomal aberrations at the sampling time points.

During this period, Wu Qingguo also came to the laboratory every other day, first to deliver mice to Tang Lou, and to observe Tang Lou's experimental process curiously, by the way, to report Tang Lou's recent situation, and Tang Lou's recording and dispensing of medicines, All kinds of beautiful photos in a daze while breathing were sent to Kim Yuna.

Finally on the seventh day, Tang Lou completed the first batch of chromosomally induced ICR mice with stable traits.

Under the arrangement of Tang Lou, Wu Qingguo sent the first batch of male parents to the experimental base and began to breed the first inbred line.

At the same time, in the research institute affiliated to "Cell", Alex mobilized all the researchers of the research institute, and at the same time called the heads of various projects, suspended the projects in hand, and began to verify Tang Lou's thesis.

(End of this chapter)