My plug-in is not that strong

Chapter 16 Pre-Experiment

【Yes, dear.You must pay the shipping fee when you use coins in a certain treasure.Because the currency is Xiaoye's energy source, and the freight paid is actually used by Xiaoye, which is converted into energy for the transportation of goods.Of course, the coins in your account are also the energy source of Xiaoye, but the amount provided is too small and can only be absorbed slowly. ] Xiaoye explained: 【It's like there is a plate of stir-fried vegetables here, Xiaoye can fill you up and replenish your energy.But by smelling the taste, the small leaves can also gain a little energy, and the vegetables are not lost. 】

Oh~ Song Yu couldn't help but think of the scene when Ludong's hometown celebrates the New Year, inviting the ancestors home, offering them on the memorial tablet, and putting tribute on the table in front of them.

In front of Xiaoye, it is better to put fried coins, fried coins, fried coins, steamed coins and boiled coins.Put another incense burner on fire.Uh, it feels like it’s the God of Wealth?
Song Yu shook his head and returned to the topic.

[No wonder you always stare at my money, it's equivalent to eating.But I can also earn a lot of coins, so you can smell more.Quantitative change produces qualitative change. 】

It is impossible for Song Yu to spend more money to buy things!Impossible in this life!Saving more money is the kingly way.

【Mr. Song, don't blame Xiaoye for talking about you.You ~ can ~ really ~ pick! ] Xiaoye's contemptuous eyes.jpg
Ha ha, even the expression pack is ready.Could it be that the degree of intimacy has increased a little, her personality has changed, and she has begun to despise me.However, Xiaoye in the picture is a little beauty, she looks like a loli, I don't know where she collected the character information.

[Fortunately, Xiaoye's energy recovery speed can be improved. ] Xiaoye proudly said: 【This depends on Mr. Song's level, authority, balance and the degree of intimacy between Xiaoye and Mr. Song.The higher the improvement of these factors, the faster the energy recovery of the leaflet will be. 】

[Great, so our goals are the same. 】Song Yu said happily.

Xiaoye gives you a supercilious look.jpg
Song Yu: ...

Can you speak well, you have always despised me.Diligence and thrift are the traditional virtues of the Chinese nation.Besides, we are on the same front. I will work hard to upgrade and earn coins, which will also increase your recovery speed.Maybe one day, your speed can be satisfied by absorbing coins alone, so can you give me free shipping.Hahahahaha
[Speaking of shipping costs, are you guys going to be very embarrassing in the future? The shipping fee of 0.5 coins is already 5 small things.It is said that it should account for 20% of the daily harvest coins. ] Song Yu asked inadvertently.

[No, dear.They almost don’t charge shipping~] Xiaoye said.

[No charge!Why, everyone bought a certain treasure, so why treat it differently! 】Song Yu said angrily.

And what's with your slightly happy tone!Could it be that intimacy also enabled you to activate the heart-pricking function?

[Mr. Song, you know.You do not belong to the future, but a certain treasure is a platform belonging to the future.The energy consumption of transporting items across the time tunnel is very high. Of course, the shipping price is related to the items you buy.But in the same time dimension, it is much simpler to transport items between three dimensions.Little leaf does not consume much energy at all. 】 Xiaoye said.

【All right.After all, I don't belong to the humans over there.You must be very lucky to have it.It should be enough, the shipping fee should not be considered. 】

Song Yu looked at the time and it was time for dinner.

After having dinner in the cafeteria, Song Yu went directly to the laboratory on the third floor.

He wanted to do a preliminary experiment first.By the way, try Xiaoye's ability.

Because the research group’s previous field experiment was carried out in a certain province in Northeast China, a small part of the saline-alkali soil collected at that time was stored in a freezer at minus 80 degrees.Such low temperatures can make microorganisms go dormant, reduce their activity, and maintain the original microbial community structure.Belongs to frozen samples.

Take out about 10g of frozen saline-alkali soil, put it in a petri dish, add 10ML of distilled water, and let it thaw at room temperature.

While waiting, Song Yu found air-dried samples of the same field soil, as well as unused corn stalks left by the brothers in the research group. These stalks had been crushed, and the bags were marked with 'screened 70 mesh' typeface.

Seeing that the soil was almost thawed, Song Yu said to Xiaoye: [Xiaoye, can you see that there are microorganisms that are highly resistant to salt and alkali in this soil? 】

Xiaoye scanned subconsciously, and quickly said: [Yes, Mr. Song.Most of the microorganisms contained in it have a certain degree of salt-alkali tolerance, and several of them have a high degree of salt-alkali tolerance.However, microbial activity in freshly thawed soil is very low and requires cultivation to activate. 】

【Of course, leaflets can be activated to add value, but the organic matter in the soil will be consumed very quickly.Need Xiaoye's help? 】 Xiaoye added.

Song Yu thought that it was going to be cultivated in a constant temperature incubator. Hearing what Xiaoye said, he quickly said: [Need!However, as long as you activate those types with high salt-alkali resistance, and control a certain amount, you don’t need too much. 】

According to Song Yu's request, Xiaoye only activated the microorganisms that she thought were highly resistant to salt and alkali, making them multiply at a tenfold rate.

【OK.Please wait for 5 minutes. 】

During these 5 minutes, Song Yu took out several 50g air-dried samples into a plastic box for cultivation, and added 70g of 5-mesh crushed straw.

The amount of straw is already very large. Song Yu wants to use the straw as a carbon and nitrogen source to support the survival of microorganisms, so as to observe the improvement effect of these microorganisms on saline-alkali soil, so as to select suitable strains for inoculation with ferric humate in later experiments.

Moreover, it is just a pre-experiment, and it does not have to simulate the amount used in the field experiment, nor does it need to be too careful.

After 5 minutes, wait for the leaflet to increase its value.Song Yu asked Xiaoye to separate these highly saline-alkali-tolerant microorganisms, suck them out with a disposable straw, and inject them into petri dishes with saline-alkali soil and straw.Then, after stirring evenly, put it into a constant temperature incubator at 25 degrees.

After all the culture boxes are placed in the incubator, the next step is the culture period.

【Mr. Song, do we need to keep the growth rate of these microorganisms at this level? 】 Xiaoye asked.

宋玉想了想，他看的相关文献上，是要培养1天、3天、5天、7天、15天、30天甚至更久的。不过他下周已经计划回家了，所以时间上不允许他培养这么久了。

[Yes, let's keep the rapid proliferation of these specific bacteria. ] Song Yu replied.

Since time does not allow, let's look at the results of 24 hours, 48 ​​hours and 72 hours first.With the multiplication buff added by Xiaoye, and there is enough organic matter for it to consume, the effect should be several times faster.

After clearing the lab table, Song Yu returned to the office.

[I don't know how the effect of the cultivation experiment will be.My expertise in microbiology is not enough.Still have to look at the literature. ] Song Yu thought.

Seeing that it was still early, Song Yu turned on the computer and began to collect literature on microorganisms.

(End of this chapter)